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nebnext ultra ii end repair da tailing module  (New England Biolabs)


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    New England Biolabs nebnext ultra ii end repair da tailing module
    Nebnext Ultra Ii End Repair Da Tailing Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+end+repair+module/NEBNext+Ultra+II+End+Repair%2FdA-Tailing+M/pmc13125923-21-0-0
    Average 99 stars, based on 1871 article reviews
    nebnext ultra ii end repair da tailing module - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification
    Article Snippet: The fragments were size selected with 0.6X NEBNext DNA Sample Purification Beads (NEB, E6178) to enrich for 1-1.5 kb DNA fragments. .. Purified DNA was end repaired with NEBNext End Repair Module (NEB, E6050) and cleaned up again with 0.6X NEBNext DNA Sample Purification Beads. .. Eluted DNA was treated with 10 units of Terminal Transferase (NEB, M0315) in a 50 mL reaction with 1 μL 9.5 mM dCTP, 0.5 mM ddCTP, 4 μL 10X TdT Buffer at 37°C for 30 minutes to add a poly-C tail to the DNA.

    Article Title: Method for determining copy number variations
    Article Snippet: Library preparation was performed using reagents of the NEBNext DNA Sample Prep DNA Reagent Set 1 (Part No. E6000L; New England Biolabs, Ipswich, MA), for Illumina as follows. .. The overhangs of approximately 2 ng purified cfDNA fragments contained in 40 μl were converted into phosphorylated blunt ends according to the NEBNext® End Repair Module by incubating in a 1.5 ml microfuge tube the cfDNA with 5 μl 10× phosphorylation buffer, 2 μl deoxynucleotide solution mix (10 mM each dNTP), 1 μl of a 1:5 dilution of DNA Polymerase I, 1 μl T4 DNA Polymerase and 1 μl T4 Polynucleotide Kinase provided in the NEBNextTM DNA Sample Prep DNA Reagent Set 1 for 15 minutes at 20° C. The enzymes were then heat inactivated by incubating the reaction mixture at 75° C. for 5 minutes. .. The overhangs of approximately 2 ng purified cfDNA fragments contained in 40 μl were converted into phosphorylated blunt ends according to the NEBNext End Repair Module by incubating in a 1.5 ml microfuge tube the cfDNA with 5 μl 10× phosphorylation buffer, 2 μl deoxynucleotide solution mix (10 mM each dNTP), 1 μl of a 1:5 dilution of DNA Polymerase I, 1 μl T4 DNA Polymerase and 1 μl T4 Polynucleotide Kinase provided in the NEBNext DNA Sample Prep DNA Reagent Set 1 for 15 minutes at 20° C. The enzymes were then heat inactivated by incubating the reaction mixture at 75° C. for 5 minutes.

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification.
    Article Snippet: The fragments were size selected with 0.6X NEBNext DNA Sample Purification Beads (NEB, E6178) to enrich for 1-1.5 kb DNA fragments. .. Purified DNA was end repaired with NEBNext End Repair Module (NEB, E6050) and cleaned up again with 0.6X NEBNext DNA Sample Purification Beads. .. Eluted DNA was treated with 10 units of Terminal Transferase (NEB, M0315) in a 50 mL reaction with 1 μL 9.5 mM dCTP, 0.5 mM ddCTP, 4 μL 10X TdT Buffer at 37°C for 30 minutes to add a poly-C tail to the DNA.

    Sample Purification:

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification
    Article Snippet: The fragments were size selected with 0.6X NEBNext DNA Sample Purification Beads (NEB, E6178) to enrich for 1-1.5 kb DNA fragments. .. Purified DNA was end repaired with NEBNext End Repair Module (NEB, E6050) and cleaned up again with 0.6X NEBNext DNA Sample Purification Beads. .. Eluted DNA was treated with 10 units of Terminal Transferase (NEB, M0315) in a 50 mL reaction with 1 μL 9.5 mM dCTP, 0.5 mM ddCTP, 4 μL 10X TdT Buffer at 37°C for 30 minutes to add a poly-C tail to the DNA.

    Article Title: High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification.
    Article Snippet: The fragments were size selected with 0.6X NEBNext DNA Sample Purification Beads (NEB, E6178) to enrich for 1-1.5 kb DNA fragments. .. Purified DNA was end repaired with NEBNext End Repair Module (NEB, E6050) and cleaned up again with 0.6X NEBNext DNA Sample Purification Beads. .. Eluted DNA was treated with 10 units of Terminal Transferase (NEB, M0315) in a 50 mL reaction with 1 μL 9.5 mM dCTP, 0.5 mM ddCTP, 4 μL 10X TdT Buffer at 37°C for 30 minutes to add a poly-C tail to the DNA.

    Phospho-proteomics:

    Article Title: Method for determining copy number variations
    Article Snippet: Library preparation was performed using reagents of the NEBNext DNA Sample Prep DNA Reagent Set 1 (Part No. E6000L; New England Biolabs, Ipswich, MA), for Illumina as follows. .. The overhangs of approximately 2 ng purified cfDNA fragments contained in 40 μl were converted into phosphorylated blunt ends according to the NEBNext® End Repair Module by incubating in a 1.5 ml microfuge tube the cfDNA with 5 μl 10× phosphorylation buffer, 2 μl deoxynucleotide solution mix (10 mM each dNTP), 1 μl of a 1:5 dilution of DNA Polymerase I, 1 μl T4 DNA Polymerase and 1 μl T4 Polynucleotide Kinase provided in the NEBNextTM DNA Sample Prep DNA Reagent Set 1 for 15 minutes at 20° C. The enzymes were then heat inactivated by incubating the reaction mixture at 75° C. for 5 minutes. .. The overhangs of approximately 2 ng purified cfDNA fragments contained in 40 μl were converted into phosphorylated blunt ends according to the NEBNext End Repair Module by incubating in a 1.5 ml microfuge tube the cfDNA with 5 μl 10× phosphorylation buffer, 2 μl deoxynucleotide solution mix (10 mM each dNTP), 1 μl of a 1:5 dilution of DNA Polymerase I, 1 μl T4 DNA Polymerase and 1 μl T4 Polynucleotide Kinase provided in the NEBNext DNA Sample Prep DNA Reagent Set 1 for 15 minutes at 20° C. The enzymes were then heat inactivated by incubating the reaction mixture at 75° C. for 5 minutes.

    Sample Prep:

    Article Title: Method for determining copy number variations
    Article Snippet: Library preparation was performed using reagents of the NEBNext DNA Sample Prep DNA Reagent Set 1 (Part No. E6000L; New England Biolabs, Ipswich, MA), for Illumina as follows. .. The overhangs of approximately 2 ng purified cfDNA fragments contained in 40 μl were converted into phosphorylated blunt ends according to the NEBNext® End Repair Module by incubating in a 1.5 ml microfuge tube the cfDNA with 5 μl 10× phosphorylation buffer, 2 μl deoxynucleotide solution mix (10 mM each dNTP), 1 μl of a 1:5 dilution of DNA Polymerase I, 1 μl T4 DNA Polymerase and 1 μl T4 Polynucleotide Kinase provided in the NEBNextTM DNA Sample Prep DNA Reagent Set 1 for 15 minutes at 20° C. The enzymes were then heat inactivated by incubating the reaction mixture at 75° C. for 5 minutes. .. The overhangs of approximately 2 ng purified cfDNA fragments contained in 40 μl were converted into phosphorylated blunt ends according to the NEBNext End Repair Module by incubating in a 1.5 ml microfuge tube the cfDNA with 5 μl 10× phosphorylation buffer, 2 μl deoxynucleotide solution mix (10 mM each dNTP), 1 μl of a 1:5 dilution of DNA Polymerase I, 1 μl T4 DNA Polymerase and 1 μl T4 Polynucleotide Kinase provided in the NEBNext DNA Sample Prep DNA Reagent Set 1 for 15 minutes at 20° C. The enzymes were then heat inactivated by incubating the reaction mixture at 75° C. for 5 minutes.

    other:

    Article Title: A structure-selective endonuclease drives uniparental mitochondrial DNA inheritance
    Article Snippet: DNA was precipitated by addition of ammonium acetate (0.75 M), glycogen (∼70 μg/mL; Thermo Fisher Scientific, R0561), and 2.5 volumes of ethanol, followed by incubation at -80°C overnight.



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